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Mohammadkarim, Mokhtari-Dizaji, Kazemian, and Saberi: Hemodynamic analysis of radiation-induced damage in common carotid arteries by using color Doppler ultrasonography

### Purpose

The aim of this study was to assess vascular changes and blood flow abnormalities in the common carotid arteries of patients with head and neck cancers after external radiotherapy, using color Doppler ultrasonography.

### Methods

We studied 24 patients treated with external radiotherapy for various head and neck cancers. In order to study the acute effects of irradiation on common carotid blood flow and arterial diameter changes, color Doppler ultrasonography parameters such as peak systolic velocity, end diastolic velocity, mean velocity, systolic-to-diastolic velocity (S/D) ratio, pulsatility index (PI), resistive index (RI), and instantaneous diameter changes were evaluated before and after external radiotherapy. Additionally, the blood volume flow (VF) values in the peak systolic and end diastolic phases, as well as mean velocity, were evaluated throughout three cardiac cycles using B-mode ultrasonic image processing.

### Results

The findings showed significant changes in the S/D ratio, PI, and RI of the common carotid arteries before and after external radiotherapy (P<0.05). Moreover, a significant decrease in artery diameter and blood VF was observed after radiotherapy relative to the pretreatment values. A significant correlation was found between the blood VF values estimated using ultrasonic measurements and mathematical methods throughout three cardiac cycles.

### Conclusion

The hemodynamic parameters of the common carotid arteries changed during radiotherapy. These arterial changes may lead to late adverse effects of radiotherapy, such as ischemic strokes and ischemic attacks.

### Introduction

External radiotherapy as a single modality or in combination with chemotherapy or surgery is a common treatment for tumors of the head and neck area [1-4]. The carotid arteries are the major vessels in the neck that supply blood to the brain, face, and neck. The main goal of external radiotherapy is to deliver the maximum dose to a tumor while achieving the minimum dose to the normal tissues. Despite technical advances in treatment planning systems, such as 3-dimensional conformal radiation therapy, intensity-modulated radiation therapy, and image-guided radiation therapy, it has not been possible to eliminate radiation exposure to the carotid arteries during radiotherapy in patients with head and neck carcinomas [5].
Under clinical conditions, functional changes in the carotid arteries during procedures that affect the blood supply can be assumed to be correlated with the ultrasonic parameters of the common carotid arteries [15,16]. Noninvasive testing includes measuring the changes induced in blood dynamics with color Doppler imaging, which is a routine method of hemodynamic analysis. Additionally, automatic analysis for the calculation of instantaneous changes in vascular function using B-mode ultrasound images has been reported to be the most accurate and repeatable method for the diagnosis of blood vessel injuries [13].
To our knowledge, no study has comprehensively characterized hemodynamic parameters to evaluate the acute functional effects of neck-region radiotherapy on radiation-treated carotid arteries. In this study, the prevalence of abnormal carotid blood supply in patients exposed to head and neck external irradiation was examined by color Doppler measurements and by performing calculations with an image processing algorithm to determine quantitatively the changes in hemodynamic parameters in these subjects. Then, the relative deviation between the measured volume flow (VF) evaluated using the color Doppler system and the mean VF derived from the computational procedures was determined. Additionally, a statistical analysis of carotid VF parameter changes in the systolic and diastolic phases of the cardiac cycle was performed, and the acute influence of radiotherapy on the hemodynamic parameters of radiation-treated common carotid arteries was assessed.

### Study Population

After approval by the Medical Ethics Committee of Tarbiat Modares University, this research was performed among patients who underwent carotid-region irradiation. Over a 7-month period, from August 2015 to March 2016, a total of 24 consecutive head and neck carcinoma patients who received primary or postoperative treatment at the Cancer Institute of Imam Khomeini Hospital in Tehran were studied before and after the external radiotherapy process. All the patients were treated by 6-MV photons and an isocentric technique delivering 2 Gy per day over 5 consecutive days per week (Varian Medical Systems, Palo Alto, CA, USA). The absorbed dose to the common carotid artery was extracted from the dose plan provided to the patient. Fifteen patients were men and nine patients were women. The participants in the study were adults, and none had a history of previous stroke or vascular surgery. The demographic and clinical characteristics of the study population are summarized in Table 1. It should be noted that informed consent for the examination was obtained from each patient in written form.
Because of differences in the tumors and treatment protocols among the subjects, unequal doses were delivered to the two carotid arteries. Hence, the present study was performed on the carotid artery that received a greater radiation dose during treatment based on the location of the tumor. The tumor type and radiotherapy doses administered to subjects are shown in Table 2.

### Ultrasonography

After at least 10 minutes of rest in the supine position, the ultrasonographic examination of the common carotid artery that received a greater radiation dose during treatment, 2-3 cm beneath the bifurcation region, was carried out as described in a previous study [17]. The SSI-6000 model of the Sonoscape ultrasonography system (SSI-6000 model, Shenzhen, China) equipped with a 6-11 MHz linear transducer was employed. Ultrasonic images were obtained at focal depth of 4.4 cm. First, the cross-sectional diameter of the common carotid artery was determined for each subject (Fig. 1). Then, blood velocity parameters were extracted from the color Doppler examination, including the peak systolic velocity (Vp), end diastolic velocity (Ve), and mean velocity (VM) of the common carotid artery. The following color Doppler parameters were calculated: the pulsatility index (PI), the resistive index (RI), and the systolic-to-diastolic velocity (S/D) ratio. The arterial cross-section was extracted by B-mode images throughout three cardiac cycles, and the blood VF was reported for each subject. It is worth noting that the color Doppler settings were the same for all tests when the common carotid artery parameters were measured. While each test was performed, the probe was positioned at the lateral side of the neck on the carotid artery in the longitudinal view.
Instantaneous changes in the diameter of common carotid artery were evaluated using consecutive B-mode ultrasound images. For this purpose, sequential images of the common carotid arteries in the AVI format (65 frames per second) were transferred to a personal computer for processing. Sequential images were exported to MATLAB (ver. 7.10.0.499, Math Work Inc., Natick, MA, USA) for extraction of BMP images from the AVI movies. The peak systolic diameter (dp), the end diastolic diameter (de), and the mean-value diameter (dM) of each lumen were determined using the maximum gradient algorithm in MATLAB, which allowed the quantitative assessment of the time-dependent movements of the vascular wall from the sequential ultrasonic images obtained throughout three cardiac cycles (Fig. 2). The essential features of this algorithm have been described in detail elsewhere [18]. Blood VF at the peak systolic mode (VFp), the end diastolic mode (VFe), and the mean value of VF (VFM) were extracted using the following formulas.
$VFp=πdp22×Vp$
$VFe=πde22×Ve$
$VFM=πdM22×VM$
For assessment of the early effects of radiotherapy, color Doppler ultrasonography and B-mode ultrasonic images were acquired for each subject before radiotherapy was begun and after the last session of fraction dose delivery, as reported by Adriaenssens et al. [19].
Finally, the relative difference (∆) of the two methods of assessing VF was evaluated by comparing the calculated VF derived from the computational method (VFcal) and the routine measurement of blood VF (VFmeas), as seen in the following equation.

### Statistical Analysis

In the present study, the necessary sample size was estimated to be 10 with a confidence level of 95% and 90% power. Therefore, 24 patients (mean age, 57 years) were enrolled in this study. All subjects underwent color Doppler ultrasonography. The hemodynamic data of each patient before and after treatment that were derived from the Doppler ultrasonography system and the mathematical procedure based on the detection of instantaneous motion in the common carotid arterial walls were analyzed using the paired-sample t test to compare the baseline hemodynamic parameters with probable abnormalities in the ultrasonic Doppler parameters of blood flow following treatment. P<0.05 were considered to indicate statistical significance.

### Results

The color Doppler parameters related to blood flow velocity that were used to evaluate the acute effects of ionizing irradiation are listed in Table 3. The treated patients had significantly more lumen constriction than the baseline group based on the internal diameter measured using the Doppler ultrasonography system and the detection of instantaneous motion in the common carotid arterial wall (Table 3).
A significant increase was found in the mean velocity, S/D ratio, PI, and RI after external radiotherapy. Radiotherapy treatment in head and neck cancer patients especially significantly reduced the internal diameter and blood VF of the common carotid arteries. Additionally, the RI parameter increased more significantly than other measured parameters derived from the blood flow velocity profile. Among these parameters, no significant changes were seen in the peak systolic velocity between the baseline group and the post-treatment group (P=0.120). The treated patients had significantly greater lumen constriction than the baseline group based on the internal diameter measured using the Doppler ultrasonography system and the detection of instantaneous motion in the common carotid arterial wall (Table 3).
Therefore, the blood VF values of radiated carotid arteries were lower than the non-radiated arteries (P<0.05).
Table 4 shows the mean±standard deviation and P-values of measured and estimated blood VF. Blood VF was significantly lower in all subjects following external irradiation than at baseline. A significant difference was found between the blood flow volume in the peak systolic phase and the end diastolic phase, as well as the mean blood flow volume of the common carotid arteries after radiotherapy compared to before treatment (P<0.05).
The relative difference between the measured VFmeas, which was used as the mean VF in the clinical investigation, and the automated VFM, which was calculated using the image processing procedure, was found to be 10.63% and 10.01% in the pretreatment and radiation-treated groups, respectively. These results show that the routine measurements of VF may be accompanied by errors. However, the blood VF obtained from the clinical investigation was overestimated by 2% relative to the blood VF calculated based on the arterial diameter in the image processing method.

### Discussion

It has been reported in qualitative studies that primary radiation to the neck increases the incidence of carotid arterial stenosis and may induce various types of structural damage [15,20]. The etiology of radiation-induced carotid artery injury in the endothelium, media, and adventitia were discussed by Gujral et al. [15]. It was established in that study that early radiation damage is characterized by an inflammatory process focused on initial changes in the endothelial cells, followed by fibrosis and necrosis in the media and adventitia. However, apart from stenosis, functional injuries or changes, especially regarding the role of blood supply, in the carotid artery following external radiotherapy to the neck area have not yet been reported. In the present study, changes in the blood flow parameters of the carotid artery before and after irradiation indicated the presence of underlying vascular complications [15].
The quantification of blood VF is clinically useful because it allows the hemodynamic assessment of carotid artery stenosis and its collateral pathways and associated conditions [14,21]. With regard to changes in the blood VF parameters of patients (Table 4), non-negligible variations in VF occurred in the irradiated arteries compared with the baseline samples throughout the cardiac cycle. Since the blood VF is approximated by multiplying the luminal crosssectional area by the corresponding mean velocity of blood flow, and the luminal cross-section is proportional to d2/4, a considerable decrease of blood VF occurred in the radiation-treated vessels. Although the variation within the mean velocity after radiotherapy was statistically significant, the numerical variation of this parameter was negligible (Table 3).
The effects of irradiation on color Doppler parameters of the inferior thyroid artery have previously been evaluated in order to assess functional changes in the thyroid gland. Significant increases in the PI, RI, blood VF, and the internal diameter of the lumen were observed after the completion of external radiotherapy in comparison with the values observed before the start of irradiation [22]. It should be noted that the mechanical structure of the inferior thyroid artery is different from that of the common carotid arteries, and it seems that the differences in the behavior of hemodynamic parameters after radiotherapy are due to such structural variations.
In the present study, we observed a larger common carotid diameter and higher blood VF values in males than in females, and in old subjects and patients with a history of smoking than in young subjects and nonsmoking patients, for both the irradiated and nonirradiated carotid arteries. However, we did not observe a significant difference between the behavior of VF in the nonirradiated and irradiated carotid arteries according to age, gender, or history of smoking. Moreover, external radiation had the same influence on the hemodynamic parameters of carotid arteries with a normal intima-media dimension and carotid arteries with greater intima-media thickness. Moreover, a relatively uniform decrease in the blood VF was seen in the systolic and diastolic phases of the cardiac cycle. In addition, a significant decrease in the VF was observed using measurement-based and computational methods (Table 5). However, our results also illustrated that the VF values derived from the ultrasonography system exhibited non-negligible error.
In some previous studies [1,7,8,10,20], carotid stenosis was reported in a follow-up test conducted at least 1 year after the patient’s last radiation treatment as a late form of toxicity. In those studies, only the internal diameter of the radiation-induced vessels was evaluated, and blood supply parameters were ignored. In this study, a decrease was found in the diameter of the carotid artery in head and neck cancer patients after treatment with radiation. These results demonstrate the near-term effects of radiation-induced toxicity due to head and neck irradiation. In light of the findings of the present study, we suggest the immediate implementation of Doppler ultrasound imaging after the treatment process, especially for patients with atherosclerotic disease, based on Doppler imaging of the carotid artery prior to undergoing irradiation in order to promptly diagnose the early effects of radiation on the blood supply.
Although the present study demonstrated that hemodynamic parameters responded to radiotherapy, it has two main limitations. First, the dose delivery methodology during the treatment process was not exactly the same in all subjects, since the patients suffered from different types of head and neck tumors. Second, the long-term effects of radiotherapy on hemodynamic parameters were not investigated in this study.
In conclusion, external radiotherapy involving the head and neck region generally increases long-term survival, but may lead to carotid stenosis and failure of the blood supply in the short-term after cancer treatment. The feasibility of using Doppler ultrasonography and B-mode image processing for the objective assessment of carotid artery hemodynamics was investigated. The most interesting finding in this study was the identification of significant differences in hemodynamic parameters, especially blood VF, between baseline and post-treatment arteries throughout three cardiac cycles using an automatic computational procedure. In conclusion, it is clear that radiation-induced damage to the carotid artery can be regarded as a form of primary damage to the vessel function.

### ACKNOWLEDGMENTS

This study was approved by the Faculty of Medical Sciences, Tarbiat Modares University. This work was supported in part by the Iran National Science Foundation (INSF).

### References

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#### Color Doppler ultrasonography of the common carotid artery with a blood velocity spectrum.

The diameter of the common carotid artery was determined for each subject. The peak systolic velocity (Vp), end diastolic velocity (Ve), and mean velocity (VM) of the common carotid artery were extracted from the color Doppler examination.

#### Instantaneous changes in the internal diameter of the common carotid artery throughout three cardiac cycles, evaluated using the computational method.

The peak systolic diameter (dp), end diastolic diameter (de), and mean value diameter (dM) of each lumen were determined from sequential ultrasonic images throughout three cardiac cycles.
##### Table 1.
General characteristics of patients
Characteristic No. (%)
Age, mean土SD (yr) 57±15
Male sex 15 (62.5)
Female sex 9 (37.5)
Vascular disease 4 (16.7)
High cholesterol 4 (16.7)
Hypertension 3 (12.5)
Tobacco use 5 (20.8)
Chemotherapy 9 (37.5)
##### Table 2.
Patient characteristics Patient Dose (Gy)
Tumor site
Oral cavity 9 (25.0) 57±7
Larynx 8 (20.8) 70±0
Parotid 2 (8.3) 50±14
Lymphoma 2 (8.3) 40±0
Oropharyngeal region 1 (4.2) 70±0
Glomus 1 (4.2) 50±0
Thyroid 1 (4.2) 20±0
Mean dose of all subjects 59±11

Values are presented as number (%) or mean±SD.

##### Table 3.
Lumen diameter and blood flow velocity parameters of the common carotid arteries before and after external radiotherapy
Peak systolic velocity (cm/sec) 47.80±9.72 55.26±13.24 0.12
End diastolic velocity (cm/sec) 12.83±2.68 11.09±3.64 0.025
Mean velocity (cm/sec) 22.88±4.57 23.3±5.18 0.035
Pulsatility index 1.58±0.37 1.78±0.43 0.027
Resistive index 0.73±0.06 0.79±0.05 <0.001
Systolic to diastolic velocity ratio 4.04±0.98 4.88±1.31 0.010
dmeas (mm) 6.12±0.54 5.26±0.52 <0.001
dp (mm) 6.42±0.67 5.63±0.67 <0.001
de (mm) 5.56±0.58 4.92±0.55 <0.001
dM (mm) 6.05±0.64 5.29±0.60 <0.001

Lumen diameter parameters were obtained by two methods: a clinical investigation (dmeas) and the automated diameter that was calculated using the image processing procedure (dp, de, dM).

##### Table 4.
Blood VF before and after external radiotherapy obtained using two methods: clinical investigations (VFmeas) and calculated using the image processing (VFp, VFe, and VFM)
Blood VFmeas (mL/min) 395.20±36.39 313.79±101.10 <0.001
Blood VFp (mL/min) 926.18±218.42 778.16±266.20 0.003
Blood VFe (mL/min) 187.62±52.61 125.63±46.11 <0.001
Blood VFM (mL/min) 387.94±105.80 306.39±85.48 <0.001

VF, volume flow.

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